Review



mouse monoclonal anti human pparγ2  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    R&D Systems mouse monoclonal anti human pparγ2
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
    Mouse Monoclonal Anti Human Pparγ2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pp+k8450b+00/Human+PPAR+gamma+2%2FNR1C3+Antibody+(Clone+K8450B)/pmc05724174-56-39-52
    Average 90 stars, based on 2 article reviews
    mouse monoclonal anti human pparγ2 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Unique Interactome Network Signatures for Peroxisome Proliferator-activated Receptor Gamma (PPARγ) Modulation by Functional Selective Ligands "

    Article Title: Unique Interactome Network Signatures for Peroxisome Proliferator-activated Receptor Gamma (PPARγ) Modulation by Functional Selective Ligands

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1074/mcp.RA117.000308

    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged PPARγ2 transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
    Figure Legend Snippet: Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged PPARγ2 transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.

    Techniques Used: Control, SDS Page, Labeling, Transfection

    Related Articles

    Immunostaining:

    Article Title: Chemerin, a Novel Peroxisome Proliferator-activated Receptor ? (PPAR?) Target Gene That Promotes Mesenchymal Stem Cell Adipogenesis
    Article Snippet: .. Immunostaining of the blots was performed using goat anti-chemerin (1:1000) (catalogue number AF2325, R&D Systems) or mouse anti-adiponectin (1:5000) (catalogue number MAB3604, Chemicon), mouse anti-cyclin A2 (1:1000) (catalogue number AF5999, R&D Systems), mouse anti-cyclin B2 (1:1000) (catalogue number AF6004, R&D Systems), mouse anti-cyclin D2 (1:1000) (catalogue number AF4196, R&D Systems), mouse anti-PPARγ (1:1000) (catalogue number PP-K8450B-00, R&D Systems), and mouse anti-β-actin (1:1000) (catalogue number ab8226, Abcam). .. The appropriate secondary antibodies conjugated with a 680 nm infrared fluorophore (LI-COR Biosciences, Lincoln, NE) were used at 1:10,000 dilution.

    Article Title: Chemerin, a Novel Peroxisome Proliferator-activated Receptor γ (PPARγ) Target Gene That Promotes Mesenchymal Stem Cell Adipogenesis
    Article Snippet: .. Immunostaining of the blots was performed using goat antichemerin (1:1000) (catalogue number AF2325, R&D Systems) or mouse anti-adiponectin (1:5000) (catalogue number MAB3604, Chemicon), mouse anti-cyclin A2 (1:1000) (catalogue number AF5999, R&D Systems), mouse anti-cyclin B2 (1:1000) (catalogue number AF6004, R&D Systems), mouse anti-cyclin D2 (1:1000) (catalogue number AF4196, R&D Systems), mouse anti-PPAR (1:1000) (catalogue number PP-K8450B-00, R&D Systems), and mouse anti- -actin (1:1000) (catalogue number ab8226, Abcam). .. The appropriate secondary antibodies conjugated with a 680 nm infrared fluorophore (LI-COR Biosciences, Lincoln, NE) were used at 1:10,000 dilution.



    Similar Products

    mouse monoclonal anti human pparγ2  (R&D Systems)
    90
    R&D Systems mouse monoclonal anti human pparγ2
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
    Mouse Monoclonal Anti Human Pparγ2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pp+k8450b+00/Human+PPAR+gamma+2%2FNR1C3+Antibody+(Clone+K8450B)/pmc05724174-56-39-52
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti human pparγ2 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    mouse monoclonal anti human ppar 2  (R&D Systems)
    90
    R&D Systems mouse monoclonal anti human ppar 2
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
    Mouse Monoclonal Anti Human Ppar 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pp+k8450b+00/Human+PPAR+gamma+2%2FNR1C3+Antibody+(Clone+K8450B)/10__1074_slash_mcp__ra117__000308-42-0-14
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti human ppar 2 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    mouse anti human ppar γ  (R&D Systems)
    90
    R&D Systems mouse anti human ppar γ
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
    Mouse Anti Human Ppar γ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pp+k8450b+00/Human+PPAR+gamma+2%2FNR1C3+Antibody+(Clone+K8450B)/pmc03430109-67-25-29
    Average 90 stars, based on 1 article reviews
    mouse anti human ppar γ - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    anti pparγ antibody  (R&D Systems)
    90
    R&D Systems anti pparγ antibody
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
    Anti Pparγ Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pp+k8450b+00/Human+PPAR+gamma+2%2FNR1C3+Antibody+(Clone+K8450B)/pmc03129180-175-5-10
    Average 90 stars, based on 1 article reviews
    anti pparγ antibody - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    mouse anti pparγ  (R&D Systems)
    90
    R&D Systems mouse anti pparγ
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
    Mouse Anti Pparγ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pp+k8450b+00/Human+PPAR+gamma+2%2FNR1C3+Antibody+(Clone+K8450B)/pmc03129180-156-50-56
    Average 90 stars, based on 1 article reviews
    mouse anti pparγ - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged PPARγ2 transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Unique Interactome Network Signatures for Peroxisome Proliferator-activated Receptor Gamma (PPARγ) Modulation by Functional Selective Ligands

    doi: 10.1074/mcp.RA117.000308

    Figure Lengend Snippet: Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged PPARγ2 transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.

    Article Snippet: Primary mouse monoclonal anti-human PPARγ (sc-7273; 408–505; Lot#: L3013) for IP, rabbit polyclonal anti-human PPARγ (sc-7196; 8–108; Lot#: A2512) for Western blot (WB), and mouse IgG (sc-2025; Lot#: C1915) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA) Mouse monoclonal anti-human PPARγ2 (PP-K8450B-00; 1–28; Lot#: A-2) for WB was obtained from R&D Systems (Minneapolis, MN).

    Techniques: Control, SDS Page, Labeling, Transfection